I take my two young daughters to Disneyland frequently, and after standing in the same lines time and time again here are some minor time-saving hints I've come up with. I just hate standing in lines, so for me every time-saver trip is a good tip! I'm curious if other Mice-chatters have different, or better strategies.
1. Matterhorn - Early in the day (I'd say first two hours) the quere beginning over by Alice and Wonderland is always much shorter than the queue starting over by the Finding Nemo Submarine Voyage Lagoon. I'd say 50% shorter some days. However, later in the day (or when only one queue is being used due to parades) the left queue is typically shorter as you don't have to compete with guests using the handicap entrance, which competes with the right side queue).
2. Its a Small World - The queue that begins on the right and ends at the far platform is usually shorter. Moreover, the handicap loading for this ride competes with the left/near queue, so the right side usually moves quicker.
3. Jungle Cruise - The queue towards the left (close to the water) is a bit shorter, and you never end up having to sit on the center seats (but then again you are always on the left side of the boat).
4. Pirates of the Carribean - The queue that heads off to the right side of the courtyeard and enters the main doors through the right is a tad bit shorter.
5. Autopia - Always wait until 3 or 4 lanes are being used before riding (even with a Fastpass). Often only 1 of the 2 lanes on the right (or near) staircase are being used. Check for this and always veer to the left (or far) stairway if only one lane is open as the far line then moves twice as fast.
6. Big Thunder Railroad - The handicap entrance for this ride competes with the trains that load from the right side of the dock (or staircase). When guests with handicap passes are waiting, CMs typically save three rows of each train the loads on the right. Often there isn't a choice of which line to join on this ride, but if there is head to the left.
7. Casey Junior Circus Train - I only take my kids on this ride if two trains are in operation, as it cuts the waiting time down by 50%. This is usually on busy days.